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  • Title: Transgenic mice harboring Aujeszky's disease virus gD gene.
    Author: Geisler V, Ernst E, Eloit M, Adam M, Marchandise J, Grobet L, Hanset R, Dessy-Doizé C, Pastoret PP.
    Journal: Acta Vet Hung; 1994; 42(2-3):353-8. PubMed ID: 7810430.
    Abstract:
    Plasmid pMLP10 gp50.5 (gD) (Eloit et al., 1990) provided the sequence to integrate. The construct has been injected into the male pronucleus of one-cell mouse embryos (CBA/C5B1). A total of 1,567 microinjected embryos were transferred into the oviduct of pseudopregnant females (Hogan et al., 1986). PCR revealed that 3 out of 10 mice were born with an integrated construct; among them, 2 mice have integrated the construct in their gonads because 20 out of 107 (18.7%) of their offspring were also transgenic. This low percentage could be explained by germline mosaicism and/or differential mortality between transgenic and non-transgenic embryos.
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