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  • Title: Anomalous migration of PCR products using nondenaturing polyacrylamide gel electrophoresis: the amelogenin sex-typing system.
    Author: Eng B, Ainsworth P, Waye JS.
    Journal: J Forensic Sci; 1994 Nov; 39(6):1356-9. PubMed ID: 7815018.
    Abstract:
    Sex-typing of biological samples can be accomplished using the polymerase chain reaction (PCR) to amplify DNA sequences that are specific for the Y-chromosome. One such system is based on PCR amplification of the X-chromosome amelogenin gene and the amelogenin-like sequences located near the centromere of the Y-chromosome. The X and Y PCR products can be distinguished from each other on the basis of a 177 basepair (bp) insertion in the X relative to the Y. In this report, we demonstrate that the amelogenin PCR products migrate anomalously using non-denaturing polyacrylamide gel electrophoresis (ND-PAGE) as opposed to agarose gel electrophoresis or denaturing PAGE. These results may be relevant to the choice of electrophoretic system used to analyze highly polymorphic loci for individual identification.
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