These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Calcium homeostasis in the presence of fura-2 in neurons dissociated from rat nucleus basalis: theoretical and experimental analysis of chelating action of fura-2.
    Author: Tatsumi H, Katayama Y.
    Journal: J Neurosci Methods; 1994 Aug; 53(2):209-15. PubMed ID: 7823623.
    Abstract:
    Intracellular calcium ions (Ca2+) play important roles in cell functions. Measurements of intracellular calcium ion concentration ([Ca2+]i) are often made with the fura-2 fluorescence recording technique in various preparations including neurons. Fura-2 has, however, a Ca(2+)-chelating action which complicates the interpretation of experimental results. In this report the chelating action of intracellular fura-2 was studied by means of computer simulations. The chelating action of an endogenous Ca(2+)-binding protein, calmodulin, was also estimated. Furthermore, whole-cell patch-clamp recordings of calcium currents (ICa) and fura-2 microfluorimetric recordings of [Ca2+]i were simultaneously made from neurons which were acutely dissociated from the rat nucleus basalis. Since Ca2+ influx can be initiated and terminated by using the voltage-clamp technique, the relationship between Ca2+ influx and rapid [Ca2+]i increase was examined. The present theoretical evaluations and experimental results disclosed the relationship between fura-2 and endogenous Ca(2+)-binding proteins; fura-2 at low concentration (10 microM) did not substantially affect the endogenous Ca2+ buffering mechanisms, but at high concentration (200 microM) effectively buffered cytosolic Ca2+ instead of endogenous Ca2+ buffers. Calcium homeostasis in neurons is furthermore discussed.
    [Abstract] [Full Text] [Related] [New Search]