These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: PGF2-isoprostanes formed during copper-induced oxidation of low-density lipoproteins are the prostaglandins that cross-react with PGE2 antibodies.
    Author: Proudfoot JM, Beilin LJ, Croft KD.
    Journal: Biochem Biophys Res Commun; 1995 Jan 17; 206(2):455-61. PubMed ID: 7826362.
    Abstract:
    It has been previously shown that LDL oxidation can produce a prostaglandin artifact(s) which cross-reacts with PGE2 antibodies. The nature of this cross-reacting material had not been identified. In this study we have examined the products of LDL oxidation and found substances cross-reacting with both PGE2 and LTB4 antibodies. Lipid extracts of copper oxidised LDL were separated by reverse-phase HPLC and fractions cross-reacting with PGE2 antibody identified. Fractions were collected and examined by GC-MS as methyl ester TMS ether derivatives. 8-epi PGF2 alpha (8-isoprostane) was identified as one of the major metabolites eluting in fractions cross-reacting with PGE2. 8-epi-PGF2 alpha showed 4% cross-reactivity with a PGE2 antibody but very little with LTB4 or thromboxane B2 antibodies. Treatment of oxidised LDL with a specific 8-isoprostane antibody removed any detectable PGE2 levels in subsequent PGE2 RIA. The generation of cross-reacting material during LDL oxidation was not influenced by indomethacin. The generation of 8-isoprostanes by free radical oxidation of arachidonic acid in LDL may explain much of the cross-reactivity seen with PGE2 antibodies. The identity of substances cross-reacting with LTB4 have yet to be identified.
    [Abstract] [Full Text] [Related] [New Search]