These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effect of insulin and gliclazide on glucose utilization by a perfused intestine-pancreas preparation isolated from diabetic and non-diabetic rats.
    Author: Tormo MA, Gomez-Zubeldia MA, Ropero F, Campillo JE.
    Journal: Acta Diabetol; 1994 Sep; 31(3):151-5. PubMed ID: 7827354.
    Abstract:
    The effect of insulin (40 microU/ml) and gliclazide (200 micrograms/ml) on intestinal glucose metabolism was investigated by using an in vitro perfused intestine-pancreas preparation isolated from normal or streptozotocin-diabetic rats. Glucose, lactate and alanine were measured enzymatically in the portal effluent. The glucose retained by the perfused preparation was reduced (P < 0.05) in diabetic versus control rats. The portal lactate levels were not modified, but alanine portal levels were increased (P < 0.05) in diabetic versus control rats. In the diabetic rats, the level of glucose retained by the preparations was increased (P < 0.05) by the presence of insulin, and insulin plus gliclazide in the perfusate. In the presence of insulin and/or gliclazide, the portal lactate levels were not modified, but the alanine levels were reduced (P < 0.05) to normal values. In preparations from non-diabetic rats, the level of glucose retained was increased (P < 0.05) by gliclazide and insulin plus gliclazide, without modification of the portal lactate and alanine levels. In conclusion, the results show that both insulin and gliclazide increased glucose utilization by perfused intestine-pancreas preparations isolated from diabetic rats. The effect was enhanced when both substances were present simultaneously in the perfusion medium.
    [Abstract] [Full Text] [Related] [New Search]