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  • Title: [Solid phase methods of immunoenzyme analysis of the herbicides 2,4-dichlorophenoxyacetic and 2,4,5-trichlorophenoxyacetic acids].
    Author: Dzantiev BB, Zherdev AV, Moreva IIu, Romanenko OG, Sapegova LA, Eremin SA.
    Journal: Prikl Biokhim Mikrobiol; 1994; 30(6):931-9. PubMed ID: 7831275.
    Abstract:
    Competitive solid-phase enzyme immunoassays for the detection of 2,4-dichlorophenoxyacetic (2,4-D) and 2,4,5-trichlorophenoxyacetic (2,4,5-T) acids have been developed and optimized. The sensitivity of the assay is 3 ng/ml for 2,4-D and 5 ng/ml for 2,4,5-T. The time of the assay is 1.5 h. The sensitivity of the assays increases after immobilization of antibodies on staphylococcus protein A, in the case of using monovalent derivatives of antibodies, and as a result of chemical modification of hapten. The detection limit for the pesticides is 0.1 ng/ml in this case, which is close to the sensitivity of the assay for the haptens conjugated with proteins.
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