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  • Title: Detection of 4-quinolone resistance mutation in gyrA gene of Shigella dysenteriae type 1 by PCR.
    Author: Rahman M, Mauff G, Levy J, Couturier M, Pulverer G, Glasdorff N, Butzler JP.
    Journal: Antimicrob Agents Chemother; 1994 Oct; 38(10):2488-91. PubMed ID: 7840594.
    Abstract:
    To study 4-quinolone resistance, the N-terminal coding region of gyrA from nalidixic acid-susceptible and -resistant isolates of Shigella dysenteriae type 1 was amplified by PCR, cloned, and sequenced. DNA sequence analysis of gyrA from nalidixic acid-resistant isolates revealed a C-to-T transition at nucleotide position 248 leading to a Ser-83-to-Leu substitution which was absent in susceptible clinical isolates. Direct HinfI digestion of PCR-amplified DNA detected similar mutations. Thus, DNA gyrase A subunit mutation Ser-83 to Leu is implicated in 4-quinolone resistance in S. dysenteriae type 1.
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