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Title: Cloning and sequencing a putative pyrophosphate-dependent phosphofructokinase gene from Entamoeba histolytica. Author: Huang M, Albach RA, Chang KP, Tripathi RL, Kemp RG. Journal: Biochim Biophys Acta; 1995 Jan 25; 1260(2):215-7. PubMed ID: 7841199. Abstract: Pyrophosphate-dependent phosphofructokinase (PPi-PFK) gene from Entamoeba histolytica was cloned from its genomic library and sequenced. The open reading frame has 1149 bp and codes for a protein of 41.5 kDa. The deduced amino acid sequence of E. histolytica PPi-PFK has 25 to 28% identity to the PPi-PFKs from Propionibacterium freudenreichii, Naegleria fowleri and potato. The amino acid residues known to contribute to the active site of PPi-PFK from P. freudenreichii are conserved.[Abstract] [Full Text] [Related] [New Search]