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  • Title: A polyomavirus enhancer mutant confers ubiquitous high transcriptional efficiency to the SV40 late promoter.
    Author: Fimia GM, Ferreira R, Passananti C, Amati P.
    Journal: Biochem Biophys Res Commun; 1995 Feb 06; 207(1):339-47. PubMed ID: 7857287.
    Abstract:
    To identify expression plasmids with high efficiency of transcription and with a broad tissue and cell range, we have constructed a recombinant vector combining the late SV40 promoter and the polyomavirus regulatory region derived from a mutant (PyNB11/1) which displays a very wide host range. We show that these recombinant enhancer-promoters are efficient drivers for heterologous gene transcription and expression in vitro in all mouse and human cells tested. The most active combination we identified contained the mutant enhancer (PyNB11/1) in the late orientation. This construct was able to promote a high efficiency of expression without significant fluctuation between cells of different tissutal origin or different differentiative stage. A possible interpretation of these results is discussed.
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