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  • Title: Human pancreatic procolipase expressed in insect cells: purification and characterization.
    Author: Lowe ME.
    Journal: Protein Expr Purif; 1994 Dec; 5(6):583-6. PubMed ID: 7858428.
    Abstract:
    A cDNA clone encoding human pancreatic procolipase was incorporated into a recombinant baculovirus. Spodoptera frugiperda insect cells infected with the recombinant baculovirus secreted procolipase into the medium, which could be isolated in a single step by immunoaffinity chromatography. The highly purified protein reactivated human pancreatic lipase in a concentration-dependent fashion and was efficiently converted to colipase by limited trypsin digestion. This expression system is suitable for producing amounts of procolipase adequate for biophysical studies.
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