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  • Title: [Mechanism of the stimulating effect of protamines on the infectivity of DNA phages].
    Author: Moiseeva TF, Sabel'nikov AG, Avdeeva AV, Il'iashenko BN.
    Journal: Zh Mikrobiol Epidemiol Immunobiol; 1976 Apr; (3):84-7. PubMed ID: 785909.
    Abstract:
    The interaction of styrin (protamine from sturgeon milt) with E. coli spheroplasts was investigated with the aid of the fluorescent probe--5-dimethylamino-1-naphthalenesulfonyl-chloride (DNS). Not less than 40% of the total (15 mug ml-1) DNA-protamine concentration was bound to spheroplasts. A part of the protein was found in the isolated membranes and inside the cell. There was a correlation between the binding of protein with spheroplasts and its biological action. The dansylation somehow decreased the protein stimulating activity in transfection.
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