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  • Title: Differential suppression of background mammalian lysosomal beta-galactosidase increases the detection sensitivity of LacZ-marked leukemic cells.
    Author: Hendrikx PJ, Martens AC, Visser JW, Hagenbeek A.
    Journal: Anal Biochem; 1994 Nov 01; 222(2):456-60. PubMed ID: 7864372.
    Abstract:
    A method is described for the detection of Escherichia coli beta-galactosidase-expressing leukemic cells in ex vivo bone marrow samples. 4-Methylumbelliferyl-beta-D-galactopyranoside is used as a substrate in a kinetic assay. D-Galactose is used to suppress endogenous lysosomal beta-galactosidase activity, yielding a sixfold increase in sensitivity. With this assay, the detection limit is one leukemic cell per 10(4) normal bone marrow cells.
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