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  • Title: Comparison of genus- and species-specific probes for PCR detection of mycobacterial infections.
    Author: Sritharan V, Iralu JV, Barker RH.
    Journal: Mol Cell Probes; 1994 Oct; 8(5):409-16. PubMed ID: 7877637.
    Abstract:
    We describe experiments comparing use of different DNA probes to detect mycobacteria in clinical specimens after PCR. The objective was to assess correlation between results using Mycobacterium genus-specific, and species-specific M. tuberculosis probes. Given sufficient concordance, sequential use of such probes would provide a useful screening tool. An evaluation of genus-specific probes compared use of repetitive sequences in the clone pMAv17 with the 65-kDa sequences. Sensitivity was 100% for pMAv17, 93% using the 65-kDa sequence; specificity was 70% for both. We then compared M. tuberculosis-specific probes developed by us (Tb400) with IS6110 and mpt40. Sensitivity using Tb400 was 100%; using IS6110 was 97%, and using mpt40 was 50%. Specificity using Tb400 and IS6110 was 68%, and was 70% using mpt40. Fourteen specimens which were PCR-positive and culture-negative, were positive using both genus probes, and the M. tuberculosis-specific probes Tb400, and IS6110. Ten of these were positive using mpt40.
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