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  • Title: Inhibitory and augmentative effects of oestradiol on LH-RH-induced release of LH by anterior pituitary glands from intact female rats in vitro.
    Author: De Koning J, van Dieten JA, van Rees GP.
    Journal: Mol Cell Endocrinol; 1976 Oct; 5(5):321-30. PubMed ID: 789157.
    Abstract:
    Anterior pituitary glands from intact dioestrous female rats were incubated in vitro and the LH released into the medium was measured at hourly intervals. The effect of a supramaximally active dose of LH-RH was first inhibited and later augmented by oestradiol added to the medium. The augmented response also occurred when LH-RH was absent during the preceding phase of the incubation. Both effects of oestradiol could be blocked by cycloheximide, which agent, however, also partly inhibited the response to LH-RH.
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