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  • Title: Bovine leukocyte adhesion deficiency in Danish Holstein-Friesian cattle. I. PCR screening and allele frequency estimation.
    Author: Jørgensen CB, Agerholm JS, Pedersen J, Thomsen PD.
    Journal: Acta Vet Scand; 1993; 34(3):231-6. PubMed ID: 7906090.
    Abstract:
    A screening program for bovine leukocyte adhesion deficiency (BLAD) in Danish Holstein-Friesian cattle has been initiated. During the first months 1611 animals were tested by a PCR based assay. Of these animals 1256, 346, and 8 were assigned normal, BLAD carriers, and BLAD affected animals, respectively. One bull, born as a co-twin, showed weak reaction for the BLAD allele on DNA isolated from leukocytes, but a normal genotype on DNA isolated from semen. Chromosome analysis showed that this bull was a blood chimaera. Estimation of the BLAD allele frequency upon the PCR test results showed that around 450 Danish calves born in 1991 might have been affected with the recessive disorder.
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