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  • Title: Isolation and sequence determination of porcine class II DRB alleles amplified by PCR.
    Author: Våge DI, Olsaker I, Lingaas F, Lie O.
    Journal: Anim Genet; 1994 Apr; 25(2):73-5. PubMed ID: 7912051.
    Abstract:
    The first domain exon of a porcine DRB gene was amplified by the polymerase chain reaction (PCR), and the nucleotide sequence was determined. In a material consisting of 10 unrelated animals, five different alleles were identified, all probably belonging to a single locus designated DRB1. In addition, a non-expressed locus, designated DRB2, was coamplified with DRB1. This pseudogene, containing a single base deletion, also exhibited some variation, but at a very restricted level compared with DRB1. In pairwise comparisons of DRB1 alleles, the number of amino acid substitutions ranged between 6 and 21 out of 83 positions compared.
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