These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Rapid identification of the common apo E isoform genotype using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP).
    Author: Aozaki R, Kawaguchi R, Ogasa U, Hikiji K, Kubo N, Sakurabayashi I.
    Journal: Mol Cell Probes; 1994 Feb; 8(1):51-4. PubMed ID: 7913206.
    Abstract:
    Polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) was used to identify the common apoprotein E (apo E) isoform genotypes. DNA from the target sequence on apo E was amplified by PCR from peripheral blood genomic DNA. Non-radiolabelled natural PCR products were electrophoresed on a polyacrylamide gradient gel and automatically silver-stained. Bands compatible with each of the three common apo E isoforms were obtained. This method, which does not require restriction enzymes or DNA purification, is a rapid and useful means of detecting the apo E isoform genotype.
    [Abstract] [Full Text] [Related] [New Search]