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  • Title: Inhibition of metal-catalyzed oxidation systems by a yeast protector protein in the presence of thiol.
    Author: Kwon SJ, Park JW, Kim K.
    Journal: Biochem Mol Biol Int; 1994 Mar; 32(3):419-27. PubMed ID: 7913363.
    Abstract:
    A protector protein from Saccharomyces cerevisiae prevented the inactivation of enzyme and oxidative damage to protein and DNA caused by a thiol/Fe3+/O2 metal-catalyzed oxidation (MCO) system but not when thiol was replaced by ascorbate. In the presence of a reduced thiol such as dithiothreitol and reduced glutathione, however, the protector protein prevented inactivation of E. coli glutamine synthetase against a MCO system comprised of ascorbate and Fe3+. The protector protein also inhibited the fragmentation of protein, incorporation of carbonyl groups into protein, strand breaks in pBluescript plasmid DNA, and the formation of 8-hydroxydeoxyguanosine in calf thymus DNA when induced by either the thiol/Fe3+ system or the ascorbate/Fe3+ system supplemented with dithiothreitol. These results suggest that antioxidant activity of protector protein against a MCO system requires thiol as a reducing equivalent to restore its catalytic activity.
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