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  • Title: Changes in cytosolic sodium caused by a toxic glutamate treatment of cultured hippocampal neurons.
    Author: Pinelis VG, Segal M, Greenberger V, Khodorov BI.
    Journal: Biochem Mol Biol Int; 1994 Mar; 32(3):475-82. PubMed ID: 7913364.
    Abstract:
    Changes in cytosolic Na+ ([Na+]i) caused by a toxic glutamate (GLU) or NMDA treatment of cultured hippocampal neurons were monitored by using SBFI fluorescent probe and imaging microscopy. Both GLU and NMDA (50 or 100 microM in Mg(2+)-free solution, 15 min) induced a marked increase in [Na+]i (from 6-8 to 30-45 mM) which persisted after the termination of a treatment. The competitive NMDA antagonist, APV (100 microM) when applied in the post-NMDA period failed to decrease the elevated [Na+]i. The results obtained strongly suggest that the main reason for an impairment of Na+/Ca2+ exchange in the post-glutamate period (see Febs Letters 1993, 324, 271-273) is a reduction of the transmembrane Na+ gradient caused apparently by inhibition of Na(+)-K+ pump.
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