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  • Title: Absence of calcitriol-mediated nongenomic actions in isolated intestinal cells of the damara mole-rat (Cryptomys damarensis).
    Author: Buffenstein R, Sergeev IN, Pettifor JM.
    Journal: Gen Comp Endocrinol; 1994 Jul; 95(1):25-30. PubMed ID: 7926652.
    Abstract:
    Calcitriol [1,25(OH)2D3] actions on intestinal calcium transport involve genomic and nongenomic pathways. Whether nongenomic 1,25(OH)2D3-mediated actions are employed was investigated using isolated intestinal epithelial cells of naturally vitamin D-deficient underground-dwelling damara mole-rats (Cryptomys damarensis). 1,25(OH)2D3-mediated nongenomic pathways of intestinal calcium uptake, measured by opening of 1,25(OH)2D3-activated voltage-sensitive Ca2+ channels (VSCC), did not occur. Rapid (1 min) 45Ca2+ transmembrane influx in intestinal cells was not significantly increased by the addition of 1,25(OH)2D3 (at concentrations from 10(-12) to 10(-6) nM), when compared to opening of VSCC in the presence of a depolarizing (elevated K+) buffer. Furthermore, even after 30 min calcium uptake was not significantly enhanced by the hormone. These findings support earlier reports that duodenal calcium absorption is independent of vitamin D and is a highly adaptive feature of a subterranean existence.
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