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  • Title: Template-dependent, in vitro replication of rotavirus RNA.
    Author: Chen D, Zeng CQ, Wentz MJ, Gorziglia M, Estes MK, Ramig RF.
    Journal: J Virol; 1994 Nov; 68(11):7030-9. PubMed ID: 7933085.
    Abstract:
    A template-dependent, in vitro rotavirus RNA replication system was established. The system initiated and synthesized full-length double-stranded RNAs on rotavirus positive-sense template RNAs. Native rotavirus mRNAs or in vitro transcripts, with bona fide 3' and 5' termini, derived from rotavirus cDNAs functioned as templates. Replicase activity was associated with a subviral particle containing VP1, VP2, and VP3 and was derived from native virions or baculovirus coexpression of rotavirus genes. A cis-acting signal involved in replication was localized within the 26 3'-terminal nucleotides of a reporter template RNA. Various biochemical and biophysical parameters affecting the efficiency of replication were examined to optimize the replication system. A replication system capable of in vitro initiation has not been previously described for Reoviridae.
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