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Pubmed for Handhelds

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  • Title: CCD camera imaging for the chemiluminescent detection of enzymes using new ultrasensitive reagents.
    Author: Akhavan-Tafti H, Schaap AP, Arghavani Z, DeSilva R, Eickholt RA, Handley RS, Schoenfelner BA, Sugioka K, Sugioka Y.
    Journal: J Biolumin Chemilumin; 1994; 9(3):155-64. PubMed ID: 7942120.
    Abstract:
    We have designed and constructed an inexpensive imaging system based on charge-coupled device (CCD) technology and utilized it to demonstrate the sensitivity and rapid detection possible with Lumigen chemiluminescent reagents. We also report the development of two new chemiluminescent reagents, Lumi-Phos Plus and Lumigen PS. Lumi-Phos Plus is an enhanced formulation for the rapid detection of alkaline phosphatase on membranes and in solution. It provides excellent images in blotting applications with exposures of under a minute. Lumigen PS represents a new generation of peroxidase detection reagents. The wide dynamic range with excellent linearity, higher signal and lower background than other chemiluminescent reagents make Lumigen PS of unsurpassed value in enzyme-linked immunoassays and nucleic acid probe assays using HRP conjugates.
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