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  • Title: [Enzymatic degradation of cell walls of yeast cells at different growth phases].
    Author: Tiunova NA, Kobzeva NIa.
    Journal: Mikrobiologiia; 1976; 45(5):781-6. PubMed ID: 794639.
    Abstract:
    The enzymes of Actinomyces cellulosae were fractionated by precipitation with ammonium sulphate and gel filtration on Sephadex G-75. Fractionation yielded protease, beta-1,3-glucanase and an enzyme of a low molecular weight and unknown nature involved in lysis by 70% of yeast cells at early exponential growth phase without the participation of the other two enzymes. The protease did not accomplish lysis of the cells; beta-1,3-glucanase was involved in degradation of the cell walls of old yeast cells.
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