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  • Title: Typing of the granulocyte-specific NA antigens by restriction fragment length polymorphism analysis.
    Author: Stein EL, Bux J, Santoso S, Mueller-Eckhardt C.
    Journal: Br J Haematol; 1994 Jun; 87(2):428-30. PubMed ID: 7947295.
    Abstract:
    An RNA-based method has been developed to genotype donors for the granulocyte-specific alloantigens NA1 and NA2. mRNA was isolated from granulocytes, reversely transcribed into cDNA and amplified using an Fc-gamma-receptor III-1 sequence-specific primer in the polymerase chain reaction (PCR). PCR products were analysed by restriction fragment length polymorphism (RFLP) using the restriction endonuclease Taq I, which provided a distinct restriction fragment pattern corresponding to the NA alleles. 17 donors were typed by PCR-RFLP and the results were in close accordance with those obtained by serological phenotyping by granulocyte immunofluorescence and the antigen capture assay MAIGA.
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