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  • Title: Monoclonal antibodies against goldfish (Carassius auratus) immunoglobulin: application to the quantification of immunoglobulin and antibody-secreting cells by ELISPOT and seric immunoglobulin and antibody levels by ELISA in carp (Cyprinus carpio).
    Author: Siwicki AK, Vergnet C, Charlemagne J, Dunier M.
    Journal: Vet Res; 1994; 25(5):458-67. PubMed ID: 7951348.
    Abstract:
    Monoclonal antibodies (mAbs) raised against heavy and light chains of goldfish immunoglobulin (Ig) were characterized by a Western blot technique. A complete cross-reactivity was observed between carp and goldfish Ig. These mAbs were used for the quantification of carp Ig and anti-Yersinia ruckeri antibodies by ELISA. An ELISPOT assay was also developed in carp to quantify Ig-secreting cells (ISC) and antibody-secreting cells (ASC). The number of ASC was maximum on day 18 post-vaccination and decreased to the basal level on day 28. The antibody levels in sera were maximum on day 18 and slowly decreased until day 28.
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