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  • Title: Routing of a secretory protein to the endocytic compartment in transfected Madin Darby canine kidney cells.
    Author: Rudick VL, Rudick MJ, Munoz-Medellin DA, Brun-Zinkernagel AM, Chang IF.
    Journal: Cell Mol Biol Res; 1993; 39(8):773-88. PubMed ID: 7951416.
    Abstract:
    Transfected Madin Darby canine kidney (MDCK) cells (3A) expressing human growth hormone (hGH) contain twice as many Golgi stacks as untransfected cells. How MDCK cells, lacking a regulated pathway, deal with (over)expression of a protein hormone, or any exogenous protein, has not been examined in detail. Since hGH constituted 10% of total secreted proteins, it was not apparent why Golgi amplification was needed, unless some enters a nonsecretory compartment. Studies were undertaken to determine hGH fate. By using an inhibitor of protein synthesis, or by analyzing pulse labeled immunoprecipitated hGH, 20-30% of hGH was shown to remain intracellular even after 4 h. That portion might be localized in the endosome/lysosome compartment, because it is post-Golgi. Immunoelectron microscopy with antibodies against hGH, clathrin, and cathepsin D demonstrated clathrin and hGH colocalized, as did hGH and cathepsin D. The latter were found in large vesicles, but no hGH appeared in lysosomes, due to its degradation. Analysis of isolated lysosome/endosomes revealed vesicles containing both hGH and cathepsin D, but more containing only cathepsin D. Endocytosis studies suggested the 3A basolateral endosomal compartment may be more capacious than normal. Thus, 3A Golgi amplification resulted in an expanded endosome compartment to accommodate secretory protein (over)expression.
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