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  • Title: [Enhancement of toxin production and sporogenesis in Cl. tetani 471 cultures under the influence of endogenous metabolites].
    Author: Korovina VP, Chikishev EV, Vaĭsman ISh.
    Journal: Zh Mikrobiol Epidemiol Immunobiol; 1976 Oct; (10):102-6. PubMed ID: 795225.
    Abstract:
    A study was made of the dynamics of the population quantity, the delicate morphology of the bacterial cells and the toxin formation in the Cl. tetani 471 cultures grown in fluid nutrient medium with caseine hydrolysate in the industrial 100-litre reactors and under laboratory conditions. Endogenous metabolites contained in the filtrates of the periodic Cl. tetani 471 cultures which reached the phase of accelerated death were capable of enhancement of the toxin formation when introduced in the amount of up to 1 : 100 at the beginning of the exponential growth of the same cultures in the reactors. The growth of the some oligosporogenic strain in the filtrates of the periodic cultures obtained at the beginning of the stationary phase or of the accelerated death phase was characterized by a slower increase in the vegetative cell count, by enhanced spore-formation and by the second, after the initial, increase in the concentration of the microbes partially connected with the spore vegetation.
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