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  • Title: [Effects of monoclonal antibodies on phagocytosis of Entamoeba histolytica to human erythrocytes].
    Author: Cheng X, Hiroshi T, Yoshimasa K, Huang M.
    Journal: Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi; 1994; 12(2):134-7. PubMed ID: 7955164.
    Abstract:
    Two monoclonal antibodies 3F7 and 4G6 were produced respectively against plasma membrane and nucleus-cytoplasm of Entamoeba histolytica. The erythrocytophagous quantification and ability of pathogenic strain HM-1:IMSS were evaluated by microscopy immediately at 5 min and 10 min after incubation with McAb 3F7 and McAb 4G6. Sera from normal mice and monoclonal antibody TCE 04 against Trypanosoma cruzi were prepared as negative controls. The McAb 3F7 showed stronger inhibitory action on the erythrocytophagous effect of HM-1:IMSS than the McAb 4G6. The result suggested a significant inhibition (P < 0.001) on erythrocytophagous effect of amoebic trophozoites in the presence of McAb 3F7. This inhibitory action appeared to have a decreasing tendency with the elapse of incubation time.
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