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  • Title: Thermal recovery of iodopsin from its meta I-intermediate.
    Author: Imamoto Y, Imai H, Yoshizawa T, Shichida Y.
    Journal: FEBS Lett; 1994 Nov 07; 354(2):165-8. PubMed ID: 7957918.
    Abstract:
    The thermal reaction of meta I-intermediate of iodopsin (metaiodopsin I), a chicken red-sensitive cone pigment, was studied by low-temperature spectrophotometry at -20 degrees C. Irradiation of iodopsin at -20 degrees C produced metaiodopsin I, whose absorption maximum was at about 470 nm. An incubation of metaiodopsin I at -20 degrees C resulted in a conversion to metaiodopsin II having absorption maximum at about 380 nm, as well as a concurrent formation of a red-shifted product stable at room temperature. Since the absorption spectrum and photo-reactivity of the red-shifted product were identical with those of iodopsin, the red-shifted product should be iodopsin. Thus a part of metaiodopsin I can revert to iodopsin by the thermal reaction unlike metarhodopsin I.
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