These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Mapping local and systemic symptom determinants of cucumber mosaic cucumovirus in tobacco.
    Author: Zhang L, Handa K, Palukaitis P.
    Journal: J Gen Virol; 1994 Nov; 75 ( Pt 11)():3185-91. PubMed ID: 7964627.
    Abstract:
    Cucumber mosaic cucumovirus (CMV) can be divided into two subgroups, I and II. LS-CMV and most other subgroup II strains cause a mild, systemic mottle on tobacco and can induce a necrotic etching (necrotic rings) symptom on inoculated tobacco leaves. In contrast, Fny-CMV and most other subgroup I strains cause severe, systemic mosaic symptoms on tobacco, but do not induce the necrotic etching symptom. Full-length cDNA clones of all three genomic RNAs of LS-CMV were constructed and infectious RNAs were generated from these clones. Using pseudorecombinants constructed from the infectious transcripts of LS-CMV and Fny-CMV, we found that both RNAs 1 and 2 of Fny-CMV are involved in determining the severity of systemic symptom on tobacco, and that LS-CMV RNA 3 contains the determinant for the necrotic etching symptom. Chimeras formed between Fny- and LS-CMV RNA 3 were used to demonstrate that the inducer of the necrotic etching symptoms mapped to the 5' 618 nucleotides of LS-CMV RNA 3, and required sequences in both the 5' non-translated region, as well as the 3a gene of CMV.
    [Abstract] [Full Text] [Related] [New Search]