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  • Title: Excision of cyclobutane dimers in genomic and episomal DNA in human cells.
    Author: Mitchell DL, Cleaver JE, Lutze LH.
    Journal: Photochem Photobiol; 1994 Sep; 60(3):221-4. PubMed ID: 7972372.
    Abstract:
    Direct determination has been made of cyclobutyl pyrimidine dimer induction and excision repair in an episomal SV40 DNA population in vivo. Maintaining SV40-transformed human (GM637) cells in confluent culture results in amplification of a mutant SV40 episome to high copy number. T4 endonuclease V was used to quantify the induction and repair of cyclobutane dimers in the SV40 episome and genomic DNA of the same cells. Differences in both parameters were observed; cyclobutane dimers were induced at 1.5-2-fold greater frequency in episomal DNA and excised at a reduced rate compared to genomic DNA in the host cells.
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