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  • Title: Xenobiotic oxidation during early pregnancy in man: peroxidase catalysed chemical oxidation in conceptual tissues.
    Author: Joseph P, Srinivasan SN, Kulkarni AP.
    Journal: Xenobiotica; 1994 Jul; 24(7):583-90. PubMed ID: 7975723.
    Abstract:
    1. A membrane-bound peroxidase activity was isolated and purified from pooled samples of human intrauterine tissue (embryo, placenta, amnion, chorion and uterine decidua) of 4-6 weeks of gestation. 2. The H2O2-dependent guaiacol oxidation catalysed by the purified peroxidase exhibited a specific activity of 10.82 +/- 1.81 mumol/min/mg protein. 3. The optimum conditions required for guaiacol oxidation catalysed by the peroxidase included 18 mM guaiacol, 400 microM H2O2, pH 9.0, and 2 micrograms/ml enzyme protein. The guaiacol oxidation was inhibited by KCN and NaN3 with IC50s of 17.6 and 22 microM, respectively. 4. Several other endogenous and exogenous chemicals also underwent H2O2-dependent oxidation catalysed by the purified peroxidase. These results suggest that peroxidase may be one of the major pathways of xenobiotic oxidation present in organogenesis-stage human conceptual tissues influencing the toxicity of chemicals to which the developing embryo is exposed.
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