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Title: High-performance liquid chromatography method for the determination of protein-glutathione mixed disulfide. Author: Kumari K, Khanna P, Ansari NH, Srivastava SK. Journal: Anal Biochem; 1994 Aug 01; 220(2):374-6. PubMed ID: 7978281. Abstract: We have developed an efficient and sensitive method for the quantification of picomole quantities of protein-glutathione and protein-cysteine mixed disulfides in ocular lens. Performic acid oxidation releases glutathione and cysteine from the protein-mixed disulfides as glutathione sulfonic acid and cysteic acid, respectively. The sulfonic acids are derivatized with phenylisothiocyanate, and the phenylthiocarbamyl derivatives are separated by HPLC using a reversed-phase ODS column, equilibrated with 0.14 M sodium acetate, containing 0.1% triethylamine and 6% acetonitrile.[Abstract] [Full Text] [Related] [New Search]