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  • Title: Properties of the Na+/H+ antiporter in Vibrio parahaemolyticus.
    Author: Kuroda T, Shimamoto T, Inaba K, Kayahara T, Tsuda M, Tsuchiya T.
    Journal: J Biochem; 1994 Jun; 115(6):1162-5. PubMed ID: 7982898.
    Abstract:
    The properties of the Na+/H+ antiporter in Vibrio parahaemolyticus, a slightly halophilic bacterium, were investigated using everted membrane vesicles. It appears that at least two Na+/H+ antiporters are present, one that is pH-dependent and one that is pH-independent. These two antiporters appear to correspond to the NhaA and NhaB antiporters of Escherichia coli, respectively. It seems that amiloride strongly inhibits the pH-dependent antiporter. Na+ is the best substrate for both of the two V. parahaemolyticus antiporters. Li+ is a poorer substrate and K+ is not a substrate. No K+/H+ antiport activity was detected in membrane vesicles of this organism. The Na+(Li+)/H+ antiport activity greatly increased with an increase in pH of the assay medium. pH did not affect the Km value of the Na+/H+ antiport, but it did increase the Vmax.
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