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  • Title: Rapid detection of rotavirus in faeces using a dipstick system with monoclonal antibodies and colloidal gold as marker.
    Author: Fernández D, Valle I, Llamos R, Guerra M, Sorell L, Gavilondo J.
    Journal: J Virol Methods; 1994 Jul; 48(2-3):315-23. PubMed ID: 7989446.
    Abstract:
    Rotavirus (RV) is known to be the most common cause of severe diarrhoea in infants and young children, each year leading to an estimated 800,000-900,000 deaths. RV also infects bovines and other species, with high morbidity and mortality. A rapid and simple 'naked-eye' dipstick system was developed to detect human RV in faeces, using nitrocellulose as solid phase, two monoclonal antibodies, and colloidal gold as marker. The system detects 10(4) viral particles (1-2 ng)/g of faeces. For human RV the specificity and sensitivity were 100% when compared with a commercial latex system, and 99% and 98%, respectively, when correlated with traditional RNA-PAGE, and 100% and 98% compared to an ELISA system.
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