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  • Title: Transformation of Mollicutes with single-stranded Tn4001 DNA.
    Author: Kapke PA, Knudtson KL, Minion FC.
    Journal: Plasmid; 1994 Jul; 32(1):85-8. PubMed ID: 7991677.
    Abstract:
    Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA.
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