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  • Title: [The use of a biotinated probe for the detection of the genomic RNA of the hepatitis A virus in clinical specimens].
    Author: Budiak EV, Sinagatullina NM, Karpukhin AV, Naumov VA, Mamaeva NV, Chizhikov VE, Zolotova VF, Mertvetsov NP.
    Journal: Vopr Virusol; 1994; 39(4):150-3. PubMed ID: 7998390.
    Abstract:
    A detection technique for hepatitis A virus (HAV) RNA by means of molecular hybridization using ss-biotinated DNA probe on the basis of M13 bacteriophage is described. The technique sensitivity reached 1-10 pg of control DNA or 100-500 pg of HAV. The experiments for detection of HAV carriers among the patients and contacts from foci of HAV outbreaks were carried out. A comparative analysis of the above technique and enzyme immunoassay and amplification technique was done and good coincidence of the results was demonstrated.
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