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  • Title: Lipophorin as a lipid carrier in the hemolymph of the horseshoe crab, Limulus polyphemus.
    Author: Rehn KG, Lee RF.
    Journal: Braz J Med Biol Res; 1994 May; 27(5):1135-9. PubMed ID: 8000334.
    Abstract:
    A high-density lipoprotein (HDL) was obtained from the hemolymph of Limulus polyphemus in yields generally less than 30 micrograms/ml by ultracentrifugal flotation. SDS-PAGE revealed two apolipoproteins with masses similar to those of apolipophorins (apoLp-I, 265 +/- 14 kDa; apoLp-II, 89 +/- 6 kDa). Lipid composition was different from both insect lipophorin and crustacean HDL, and showed less diacylglycerols than triacylglycerols (3.8% and 36.2% of total lipids, respectively). Since Limulus polyphemus is closely related to precambrian chelicerates, our results confirm that lipophorin was present early in the evolution of arthropods.
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