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  • Title: Cloning and sequence analysis of the gene encoding pyruvate kinase in Trypanoplasma borelli.
    Author: Ernest I, Opperdoes FR, Michels PA.
    Journal: Biochem Biophys Res Commun; 1994 Jun 15; 201(2):727-32. PubMed ID: 8003008.
    Abstract:
    The gene coding for pyruvate kinase in Trypanoplasma borelli has been cloned and characterized. A single gene copy was found with an open reading frame for a polypeptide of 496 amino acids and a molecular mass of 54337. The deduced amino acid sequence has a calculated net charge of -3. Comparison of the sequence with those of pyruvate kinases from members of the family Trypanosomatidae revealed amino acid identities of 58.4-61.5%, and, to some extent, conservation of residues supposed to be involved in the binding of the allosteric effector fructose 2,6-bisphosphate. Some kinetic properties of Trypanoplasma borelli pyruvate kinase have been determined and appear to be similar to those of the enzyme from Trypanosoma brucei and Leishmania mexicana.
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