These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Physiological roles of glycine and gamma-aminobutyric acid in dissociated neurons of rat visual cortex.
    Author: Takahashi Y, Shirasaki T, Yamanaka H, Ishibashi H, Akaike N.
    Journal: Brain Res; 1994 Mar 21; 640(1-2):229-35. PubMed ID: 8004449.
    Abstract:
    The effects of glycine (Gly) and gamma-aminobutyric acid (GABA) on the neurons acutely dissociated from rat visual cortex (VC) were investigated in the whole-cell mode using a conventional patch-clamp technique. GABA and Gly evoked Cl- currents (ICl) in a concentration-dependent manner at a holding potential (VH) of -50 mV. The half maximum effective concentrations (EC50) were 4.64 x 10(-6) M for GABA and 6.67 x 10(-5) M for Gly. Strychnine and bicuculline reversively inhibited both 10(-5) M GABA- and 10(-4) M Gly-induced ICl in a concentration-dependent manner. The half maximum inhibitory concentrations (IC50) of strychnine on GABA- and Gly-induced currents were 4.00 x 10(-6) M and 8.26 x 10(-8) M, respectively. The IC50 values of bicuculline on GABA and Gly responses were 1.18 x 10(-6) M and 2.97 x 10(-4), respectively. GABA at 10(-5) M, which is near the EC50 of the GABA response, induced ICl in all neurons tested (n = 83). However, Gly of 10(-4) M, which is also near the EC50 of the Gly response, induced ICl in 34 out of 83 neurons tested (41%). Moreover, the maximum amplitude of the Gly response was about 60% of that of the GABA response. On the other hand, the enhancement of N-methyl-D-aspartate (NMDA, 3 x 10(-4) M) response by Gly (10(-6) M) was observed in all neurons (n = 36) whether they had the Gly-induced ICl or not.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]