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  • Title: Purification of two active fusion proteins of the Na(+)-dependent citrate carrier of Klebsiella pneumoniae.
    Author: Pos KM, Bott M, Dimroth P.
    Journal: FEBS Lett; 1994 Jun 20; 347(1):37-41. PubMed ID: 8013657.
    Abstract:
    The sodium-ion-dependent citrate carrier of Klebsiella pneumoniae (CitS) was purified by means of bioengineerical methods. By fusing the biotin acceptor domain of the alpha-subunit of the oxaloacetate decarboxylase of K. pneumoniae to the C-terminus of CitS, purification of the carrier was achieved by use of a monomeric avidin-Sepharose column. Additionally, we were able to purify a CitS-protein with an N-terminal histidine-tag by immobilized metal chelate affinity chromatography (with Ni2(+)-nitrilotriacetic acid-(NTA-) resin). Both purified fusion proteins showed citrate transport activity after reconstitution into liposomes by the freeze/thaw/sonication procedure.
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