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  • Title: Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.
    Author: Menon NK, Chatelus CY, Dervartanian M, Wendt JC, Shanmugam KT, Peck HD, Przybyla AE.
    Journal: J Bacteriol; 1994 Jul; 176(14):4416-23. PubMed ID: 8021226.
    Abstract:
    The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome.
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