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  • Title: Isolation and characterization of a cDNA that encodes a novel proteinase inhibitor I from a tobacco genetic tumor.
    Author: Fujita T, Kouchi H, Ichikawa T, Syõno K.
    Journal: Plant Cell Physiol; 1993 Jan; 34(1):137-42. PubMed ID: 8025817.
    Abstract:
    We have isolated a cDNA clone, designated GTI, by screening a tobacco genetic tumor cDNA library with a tumor-specific "subtracted" cDNA probe. The cDNA contained the entire coding sequence for a 94-amino-acid polypeptide that exhibited significant homology to members of the proteinase inhibitor I family from tomato and potato. The predicted protein has a pre-sequence of 22 amino acids but lacks a pro-sequence, unlike genes for proteinase inhibitor I isolated to date. Furthermore, the protein encoded by GTI cDNA has a novel reactive site, having glutamine as the P1 reactive residue. These results suggest that the GTI protein is a novel member of the proteinase inhibitor I family. The mRNA for GTI accumulated at a high level but only transiently after the wounding of tobacco plants. Thus, it appears that the GTI protein has a function that is related to the protection of tissues against damage due to wounding.
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