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  • Title: Phorbol ester-induced potentiation of myogenic tone is not associated with increases in Ca2+ influx, myoplasmic free Ca2+ concentration, or 20-kDa myosin light chain phosphorylation.
    Author: Laporte R, Haeberle JR, Laher I.
    Journal: J Mol Cell Cardiol; 1994 Mar; 26(3):297-302. PubMed ID: 8028013.
    Abstract:
    We studied the extent of Ca2+ influx, myoplasmic free Ca2+ concentration changes, and phosphorylation of the regulatory 20-kDa myosin light chain (LC20) associated with the potentiation of stretch-induced myogenic tone in the rabbit facial vein. The protein kinase C (PKC) activator, phorbol 12-myristate 13-acetate (PMA), was used to augment Ca(2+)-dependent stretch-induced myogenic tone. Veins stretched to an optimal resting tension in physiological salt solution (PSS) containing 0.4 mM of Ca2+ developed stretch-induced myogenic tone. Tissues incubated in Ca(2+)-free PSS, either with or without PMA (0.1 microM) did not develop myogenic tone. Readmission of Ca2+ (0.4 mM) caused a three-fold increase in the contraction in PMA-treated segments (710 +/- 60 mg, n = 29 v control: 188 +/- 10 mg, n = 24). This increased contraction was not associated with additional increases in either Ca2+ influx (73.5 +/- 6.9 pmol/mg of tissue/min, n = 29 v control: 61.1 +/- 5.7 pmol/mg of tissue/min, n = 24), myoplasmic free Ca2+ concentration or LC20, (0.44 +/- 0.02 mol PO4/mol LC20, n = 9 v control: 0.43 +/- 0.03 mol PO4/mol LC20, n = 7). Our results suggest that PKC activation amplifies stretch-induced myogenic tone in the rabbit facial vein through target proteins that are not associated with regulation of Ca2+ influx, myoplasmic free Ca2+ concentration, or LC20 phosphorylation. We conclude that the PKC-mediated potentiation of stretch-induced myogenic tone is due to an increased sensitivity of the contractile apparatus to Ca2+.
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