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  • Title: [The use of pyrophosphate-dependent phosphofructokinase for the determination of fructose-2,6-bisphosphate concentration in biological material].
    Author: Golubev MA, Markova MS.
    Journal: Klin Lab Diagn; 1993; (6):27-9. PubMed ID: 8032776.
    Abstract:
    The authors discuss methods for purification of pyrophosphate fructose-6-phosphate phosphotransferase from potato tuber and enzymatic method for measuring fructose-2,6-biphosphate concentration in isolated hepatocytes of normal and diabetic rats and come to a conclusion on a higher sensitivity of the method they propose in comparison with the method making use of commercial phosphofructokinase from rabbit muscles. Their method may be used for fructose-2,6-biphosphate measurements in various biologic materials.
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