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  • Title: Polarographic assay of hydrogen peroxide accumulation in microbial cultures.
    Author: Dempsey PM, O'Leary J, Condon S.
    Journal: Appl Microbiol; 1975 Feb; 29(2):170-4. PubMed ID: 803813.
    Abstract:
    A method is described for determining low concentrations of hydrogen peroxide by using a polarographic oxygen electrode to measure the oxygen released into solution on addition of catalase. A sample can be assayed directly without prior manipulation in 3 min. The method is capable of assaying hydrogen peroxide concentrations as low as 7 muM. The method has proved extremely useful for the assay of hydrogen peroxide secreted into milk by lactic acid bacteria.
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