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  • Title: A sensitive promoter assay based on the transcriptional activator Tat of the HIV-1 virus.
    Author: Koken SE, van Wamel J, Berkhout B.
    Journal: Gene; 1994 Jul 08; 144(2):243-7. PubMed ID: 8039709.
    Abstract:
    We developed a sensitive vector system for the analysis of weak promoter activities. This promoter assay is based on the transcriptional activator protein, Tat, of human immunodeficiency virus type 1 (HIV-1). High-level expression of HIV requires activation in trans by Tat of the promoter in the long terminal repeat (LTR). Here we describe the construction of a promoterless pTat vector. Foreign promoter elements can be inserted upstream from the tat gene, and expression of Tat protein is measured in trans on a co-transfected LTR-CAT reporter plasmid. We show that this binary system is more sensitive than standard pCAT reporter assays.
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