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  • Title: UV-transparent, replaceable agarose gels for molecular-sieve (capillary) electrophoresis of proteins and nucleic acids.
    Author: Hjertén S, Srichaiyo T, Palm A.
    Journal: Biomed Chromatogr; 1994; 8(2):73-6. PubMed ID: 8044025.
    Abstract:
    Gels of methoxylated agarose (gelling point 25.6 degrees C) and other low-melting agarose derivatives compare favorably with cross-linked polyacrylamide gels for capillary and slab molecular-sieve electrophoresis of proteins and DNA. These agarose gels can be pressed out of the capillary following a run and replaced by an agarose solution with a temperature of 35-40 degrees C. Gelation occurs upon lowering temperature and the same capillary can thus be reused for another analysis with a fresh gel. The methoxylated, non UV-absorbing agarose gels are, accordingly, replaceable, which makes them very attractive for series analyses with modern, automated capillary electrophoresis apparatus. The high resolution of these agarose gels is demonstrated with a separation of an albumin sample into monomers, dimers, trimers, tetramers, pentamers, hexamers, heptamers, and of DNA fragments.
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