These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Biologically active components from mycobacterial cell walls. III. Production of experimental allergic encephalomyelitis in guinea-pigs.
    Author: Meyer TJ, Azuma I, Ribi EE.
    Journal: Immunology; 1975 Feb; 28(2):219-29. PubMed ID: 804436.
    Abstract:
    The efficacy of various fractions of mycobacterial cell walls in producing experimental ahlergic encephalomyelitis (EAE) has been evaluated. BCG (Bacillus-Calmette-Buérin) cell walls were effective in producing EAE in all animals at dose levels as low as 40 mug. Study of subfractions of these cell walls revealed the following: (1) wax D was active, but required larger doses than BCG cell walls; (2) the chloroform-methanol-soluble (CMS) portion of wax D and P3 (a mycolic acid-trehalose ester contained therein) were inactive; (3) the chloroform-methanol-insoluble (CMI) portion of wax D was active; (4) exhaustively delipidated cell wass skeletons of BCG, Nocardia asteroides, Mycobacterium smegmatis, Corynebacterium diphtheriae and M. kansaii were active; (5) two water-soluble adjuvants prepared from mycobacteria were active. These results suggest that the mycobacterial structure responsible for EAE adjuvanticity is present in the organic solvent-insoluble cell wall skeleton framework. The activity of wax D may be due to the presence of cell-wall skeleton constituents which are found in varying quanity in most wax D preparations. Wax D components soluble in a solution of chloroform:methanol (diluted 2:1 v/v) do not produce EAE.
    [Abstract] [Full Text] [Related] [New Search]