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  • Title: tRNA regions which contact with the ribosomal poly(U)-programmed P-site.
    Author: Nekhai SA, Parfenov DV, Saminsky EM.
    Journal: Biochim Biophys Acta; 1994 Aug 02; 1218(3):481-4. PubMed ID: 8049279.
    Abstract:
    Equilibrium binding affinity of yeast tRNA(Phe) for Escherichia coli poly(U)-programmed 70S ribosomal P-site was compared with corresponding affinities of several tRNA(Phe) 3'- and 5'-end-truncated derivatives, all containing the anticodon arm. Our findings strongly suggest that besides three 3'-terminal-CCA nucleotides (C74, C75 and A76), only the tRNA(Phe) anticodon arm (N28-N42) contains ribosomal P-site contact centers and that there are no such centers in the intermediate regions N1-N27 and N43-N73.
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